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- Title
Production of Foot-and-Mouth Disease Type O and A Vaccine Antigens on a Pilot Scale and Determination of Optimal Amount of Antigen for Monovalent Vaccines.
- Authors
Kim, Jae Young; Lee, Ji-Hye; Yang, Jong Min; Lee, Seo-Yong; Park, Sun Young; Jin, Jong Sook; Kim, Dohyun; Park, Jung-Won; Park, Jong-Hyeon; Park, Sang Hyun; Ko, Young-Joon
- Abstract
Foot-and-mouth disease (FMD) is a highly infectious disease affecting cloven-hoofed animals and causes significant economic losses to the livestock industry. The Type O PanAsia-2 (O PA-2) vaccine strain is protective against a wide range of serotype O FMD virus (FMDV) strains in East Asia, and A22 Iraq/24/64 (A22 IRQ) is the most widely used vaccine strain in FMD vaccine antigen banks. The aim of this study was to produce antigens from O PA-2 and A22 IRQ viruses using a 100 L bioreactor and evaluate the protective efficacy of varying antigen concentrations in pigs. More than 2 μg/mL of the antigen was recovered from the O PA-2 and A22 IRQ virus-infected supernatants. Further, inactivation of O PA-2 and A22 IRQ by binary ethyleneimine revealed that the viral titers decreased below 10−7 TCID50/mL within 13 h and 9 h, respectively. The O PA-2 and A22 IRQ vaccines, containing 10 μg and 5 μg of antigen, respectively, provided protection against homologous viruses in pigs. This is the first report demonstrating that the antigens obtained from the pilot-scale production of O PA-2 and A22 IRQ are viable candidate vaccines. These results will pave the way for industrial-scale FMD vaccine production in South Korea.
- Subjects
EAST Asia; SOUTH Korea; FOOT &; mouth disease; ANTIGENS; VACCINES; LIVESTOCK losses; ANIMAL industry
- Publication
Vaccines, 2023, Vol 11, Issue 7, p1156
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines11071156