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- Title
Effects of timolol-related ophthalmic solutions on cultured human conjunctival cells.
- Authors
Kawase, Kazuhide; Wenzhong Lin; Aoyama, Yumiko; Yamamoto, Tetsuya; Shimazawa, Masamitsu; Hara, Hideaki
- Abstract
Purpose: To investigate the inhibitory effects of drugs containing timolol on the proliferation of human conjunctival cells in vitro. Methods: Timoptol, Timoptol XE, Rysmon TG, and Timabak solutions were used. These commercially available drugs were diluted to 1/30, 1/100, and 1/300, and their effects on cell morphology, cell count, and cell activity were investigated. The effects of drugs containing benzalkonium chloride (BAK) as well as those of the Rysmon TG vehicle alone were also assessed. Results: At 1/30 dilution, cells treated with Timoptol and Timoptol XE showed cell deformation. Timoptol and Timoptol XE also caused a significant decrease in the number of cells at 1/100 and 1/30 dilutions. Cell activity decreased in a concentration-dependent manner after the addition of either Timoptol or Timoptol XE. Rysmon TG and Timabak showed significantly higher cell activity than Timoptol or Timoptol XE at both 1/100 and 1/30 dilutions. The cell count increased in a concentration-dependent manner in the BAK-free group, while cell activity decreased in a concentration-dependent manner in the cultures in the BAK-containing group. Conclusions: Compared with Timoptol and Timoptol XE, Rysmon TG and Timabak showed milder toxicity on human conjunctival cells in vitro.
- Publication
Japanese Journal of Ophthalmology, 2010, Vol 54, Issue 6, p615
- ISSN
0021-5155
- Publication type
Article
- DOI
10.1007/s10384-010-0881-2