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- Title
黄精多糖对TNF-α作用的大鼠成骨细胞增殖、凋亡影响及其机制研究.
- Authors
刘超; 徐飞; 吴涛; 王子平; 赵晓明
- Abstract
To investigate the effects of polygonatum sibiricum polysaccharide (PSP) on the proliferation and apoptosis of rat osteoblasts induced by tumor necrosis factor-α (TNF-α) and its possible mechanism. Methods MTT assay was used to detect the effect of different concentrations of PSP on TNF-α-induced osteoblast proliferation, and the appropriate concentration of PSP was screened. Flow cytometry was used to detect the effect of PSP on the apoptotic ability of osteoblasts induced by TNF-α. Real-time quantitative PCR (qRT-PCR) was used to detect the effect of PSP on the expression of miR-212 in osteoblasts induced by TNF-α. The protein expression levels of Cyclin D1, Bcl-2, Bax and P21 in osteoblasts were detected by Western blot. Results TNF-α could inhibit the proliferation of osteoblasts, promote the expression of P21 protein, and inhibit the expression of Cyclin D1 protein. The different doses of PSP could promote the proliferation of osteoblasts, the expression of Cyclin D1 was up-regulated, and the expression of P21 was down-regulated. TNF-α could promote osteoblast apoptosis, up-regulate the expression of Bax, and decrease the expression of Bcl-2 and miR-212. PSP could increase the expression level of miR-212. Up-regulation of miR-212 expression could significantly reverse the effect of TNF-α on osteoblasts. Inhibiting the expression of miR-212 could significantly reverse the effect of PSP on TNF-α-induced osteoblast proliferation and apoptosis. Conclusion Polygonatum polysaccharide can promote the osteoblast proliferation and inhibit apoptosis by up-regulating miR-212 expression.
- Publication
Chinese Journal of Osteoporosis, 2020, Vol 26, Issue 8, p1147
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2020.08.011