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- Title
Effect of kidney-tonifying, spleen-strengthening, and blood stasis removing recipe on the expression ERRα and PGC-1α mRNA in the bone marrow of the femurs in ovariectomized rat model.
- Authors
DENG Weimin; WEI Qiushi; TAN Xin; WANG Haibin; SUN Weishan; SHAO Yu
- Abstract
Objective To explore the effect of kidney-tonifying, spleen-strengthening, and blood stasis removing recipe on the expression of ERRa and PGC-1α mRNA in the bone marrow of the rat femurs in an ovariectomized rat model. Methods Forty 7-month-old female SD rats were randomly divided into sham-operated group, ovariectomized (OVX) group, herb group (kidney-tonifying, spleen-strengthening, and blood stasis removing recipe), and alendronate (ALEN) group, with 10 rats per group. After a 12-week treatment, the bone mineral density (BMD) of the left femur was measured using small animal special dual energy X-ray absorptiometry. Bone morphology of the proximal tibia was observed using HE staining. The expression of ERRa and PGC-1α mRNA of bone marrow was detected using quantitative PCR. Results Compared with that in sham-operated group, BMD in OVX group was obviously decreased (P < 0.05). Compared with that in OVX group, BMD in herb group and in ALEN group was obviously increased (P < 0.05). There was no statistically significant among sham-operated, herb and ALEN groups. Compared with sham-operated group, the expression of ERRα mRNA in OVX group was obviously increased (P < 0.05) and the expression of PGC-1α mRNA was obviously decreased (P < 0.05). After the drug intervention, the expression of ERRα mRNA in herb group was obviously decreased (P < 0.05) and the expression of PGC-1α mRNA was obviously increased compared with that in OVX group (P < 0.05). The expression of ERRα mRNA in ALEN group was obviously decreased (P < 0.01), but PGC-1α mRNA expression increased with no statistical difference (P>0.05). Conclusion The kidncy-tonifying, spleen-strengthening, and blood stasis removing recipe enhances rat BMD by increasing the expression of PGC-1α mRNA and decreasing the expression of ERRα mRNA.
- Publication
Chinese Journal of Osteoporosis, 2014, Vol 20, Issue 10, p1143
- ISSN
1006-7108
- Publication type
Article