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- Title
Ginsenoside Rg1 protects human fibroblasts against psoralen- and UVA-induced premature senescence through a telomeric mechanism.
- Authors
Zhou, Bing-rong; Xu, Yang; Wu, Di; Permatasari, Felicia; Gao, Ying-ying; Luo, Dan
- Abstract
This study was aimed to investigate the protective effects of ginsenoside Rg1 on 8-methoxypsoralen(8-MOP)/Ultraviolet A (UVA)-induced premature senescence in human fibroblasts, and the underlying mechanism. We established a stress-induced premature senescence model by 8-MOP/UVA irradiation. The aging condition was determined by histochemical staining of senescence-associated β-galactosidase (SA-β-gal). Relative telomere length was calculated by the ratio of the amount of telomere DNA versus single copy DNA by real-time polymerase chain reaction, and protein levels of p-P53, p21 and p16 were estimated by Western blotting. Compared with the 8-MOP/UVA treatment group, we found that the irradiated fibroblasts pretreated with ginsenoside Rg1 demonstrated a decrease in the expression of SA-β-gal, a downregulation in the level of senescence-associated proteins, and a deceleration in telomere shortening. Taken together, these results suggest that ginsenoside Rg1 significantly antagonizes premature senescence induced by 8-MOP/UVA in fibroblasts.
- Subjects
TELOMERES; GINSENOSIDES; FIBROBLASTS; ULTRAVIOLET radiation; HISTOCHEMISTRY; PSORALENS; CELLULAR aging
- Publication
Archives of Dermatological Research, 2012, Vol 304, Issue 3, p223
- ISSN
0340-3696
- Publication type
Article
- DOI
10.1007/s00403-012-1221-9