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- Title
The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation.
- Authors
Cao, Ting-Ting; Zhang, Yu-Qing
- Abstract
Cell culture and cryopreservation are necessary for clinical therapy and cells storage. The addition of 10% (v/v) foetal bovine serum (FBS) to basal culture media has been common practice and is one of the most widely used methods. FBS media added with 10% DMSO (dimethyl sulfoxide) have also been used for cryopreservation cells. Ideally, FBS should be avoided because of high cost and bio-safety. Silk sericin has been used as a serum substitute and an additive due to its good hydrophilicity and biological safety. This article summarizes a few details about the processing of sericin and its application as a serum substitute or an additive for cell culture and cryopreservation media. Sericin can be a potential novel serum substitute or an additive for cell culture and cryopreservation media.
- Subjects
CRYOPRESERVATION of cells; BLOOD serum analysis; CELL culture; SERICIN; DIMETHYL sulfoxide
- Publication
Amino Acids, 2017, Vol 49, Issue 6, p1029
- ISSN
0939-4451
- Publication type
Article
- DOI
10.1007/s00726-017-2396-3