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- Title
Sorbitol Accumulation in Schwann Cells Under Prolonged Hyperglycaemic Condition.
- Authors
Zaini, Fazlin; Mona, Resni; Daud, Muhamad Fauzi; Myo Than; Muhamad, Noorzaid
- Abstract
Introduction: Glucose is important for metabolic activity both in the peripheral nerve and Schwann cells. In hyperglycaemia, the polyol pathway is activated due to hexokinase saturation by the excess glucose which results in sorbitol intracellular accumulation. This study was conducted to determine the sorbitol concentration in Schwann cells following long term exposure to high glucose condition. Methods: The Schwann cell culture was grown in three different culture medium conditions (25 mM glucose, 100 mM glucose and 100 mM glucose with 1 µM epalrestat) for 14 days. At the end of the experiment, the cells were detached, and a cell count was performed. The cells were extracted, and the sorbitol concentration was determined using the sorbitol assay kit. Results: The trend of increasing sorbitol concentration in Schwann cells towards glucose concentration and its reduction due to aldose reductase inhibitor epalrestat was observed; however, this trend was not significantly different between the culture groups. Discussion: There is a possibility that sorbitol may leak into the culture medium and therefore, are less detectable in the Schwann cells. Schwann cell lines subcultured from primary Schwann cell culture of adult Wistar rat are less sensitive than immortalised adult mouse Schwann cell lines IMS32 towards high glucose induced-polyol pathway activation. Schwann cells at a lower passage number is more reliable as these cells are genetically closer to the in vivo Schwann cells compared to immortalised Schwann cells. The genetic materials alteration could have been modified in the immortalised Schwann cells to become more sensitive towards the polyol pathway. Conclusion: This suggest that intracellular sorbitol detection may be unreliable; and other parameters, such as aldose reductase measurement could be used to demonstrate polyol pathway activation by hyperglycaemia.
- Subjects
SCHWANN cells; SORBITOL; PRIMARY cell culture; REDUCTASE inhibitors; ALDOSE reductase; NEURONS; LABORATORY rats
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p42
- ISSN
1675-8544
- Publication type
Article