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- Title
Recombinant influenza viruses as delivery vectors for hepatis B virus epitopes.
- Authors
Jae-Min Song; Kwang-Hee Lee; Baik-Lin Seong
- Abstract
Purpose: Neuraminidase (NA) of influenza virus contains stalk region that shows a great deal of variability in both amino acid sequence and length. In this paper, we investigated generation of recombinant influenza viruses that had hepatitis B virus (HBV) B cell epitopes in the NA stalk region as a dual vaccine candidate. Materials and Methods: We used the WSH-HK reassortant helper virus for rescue of recombinant influenza virus containing HBV epitopes and reverse genetic protocol based on the use of micrococcal nuclease-treated virus cores for reconstitution of ribonucleoproteins. Results: We successfully generated a chimeric influenza viruses which contained 22 amino acid peptides in the stalk region derived from the surface and pre-surface protein HBV. The growth kinetics of the recombinant viruses was investigated after infection of Madin-Darby canine kidney (MDCK) and Madin-Darby bovine kidney (MDBK) cells and the rIV-BVPreS virus showed higher titer than other viruses in MDCK cells. We also confirmed the presence of HBV epitopes in the chimeric viruses by enzyme-linked immunosorbent assay (ELISA) using anti-HBV polyclonal antibody. When the ratio of recombinant virus verse wild type virus was calculated by ELISA, recombinant viruses exhibited 2 fold higher values than the wild type virus. Conclusion: These results suggest that chimeric influenza virus which contained foreign anti-gens can be used as dual vaccine against both HBV and influenza viruses.
- Subjects
INFLUENZA viruses; HEPATITIS B virus; NEURAMINIDASE; EPITOPES; VIRAL vaccines; REVERSE genetics; NUCLEOPROTEINS; ENZYME-linked immunosorbent assay
- Publication
Clinical & Experimental Vaccine Research, 2012, Vol 1, Issue 1, p77
- ISSN
2287-3651
- Publication type
Article
- DOI
10.7774/cevr.2012.1.1.77