We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Changes in Drosophila melanogaster midgut proteins in response to dietary Bowman–Birk inhibitor.
- Authors
Li, H.-M.; Margam, V.; Muir, W. M.; Murdock, L. L.; Pittendrigh, B. R.
- Abstract
The midgut proteome of Drosophila melanogaster was compared in larvae fed dietary Bowman–Birk inhibitor (BBI) vs. larvae fed a control diet. By using two-dimensional gel electrophoresis, nine differentially expressed proteins were observed, which were associated with enzymes or transport functions such as sterol carrier protein X (SCPX), ubiquitin-conjugating enzyme, endopeptidase, receptor signalling protein kinase, ATP-dependent RNA helicase and α-tocopherol transport. Quantitative real-time PCR verified differential expression of transcripts coding for six of the proteins observed from the proteomic analysis. BBI evidently affects expression of proteins associated with protein degradation, transport and fatty acid catabolism. We then tested the hypothesis that SCPX was critical for the Drosophila third instars’ response to BBI treatment. Inhibition of SCPX caused the third instars to become more susceptible to dietary BBI.
- Subjects
DROSOPHILA; PROTEINS; INSECTS; ELECTROPHORESIS; PHASE partition
- Publication
Insect Molecular Biology, 2007, Vol 16, Issue 5, p539
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.1365-2583.2007.00750.x