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- Title
Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri.
- Authors
Grotjohann, Norbert; Kowallik, Wolfgang
- Abstract
In crude extracts of Chlorella kessleri Fott and Novákóva cells grown autotrophically in white light the activity of phosphofructokinase (PFK, EC 2.7.1.11) is 62.9 ± 1.5 nmol (mg protein)-1 min-1 under optimized test conditions. It is greatly increased in red [88.3 ± 1.8 nmol (mg protein) -1 min-1], but somewhat decreased [57.0 ± 0.5 nmol (mg protein) -1 min-1] in blue light of equal productivity. Mixtures of blue and red light yield the low activity as long as blue light represents at least 35% of the total quantum fluence rate. The rough wavelength dependence of the counteracting effect of the red light exhibits a broad peak at 460nm, reminiscent of action spectra of the blue/UV photoreceptor(s). Upon transfer of red light-grown cells to blue light, the decrease develops slowly within 72 h; it cannot be prevented by 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU). Since there is less carbohydrate in blue than in red light-exposed cells, correlations between biosynthesis of PFK and level of carbohydrate are discussed, based on transport of metabolites across the chloroplasts envelope.
- Subjects
EFFECT of blue light on plants; CHLORELLA; PHOSPHOTRANSFERASES; DIURON; FRUCTOSE; CHEMICAL ecology
- Publication
Physiologia Plantarum, 1989, Vol 75, Issue 1, p43
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1989.tb02061.x