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- Title
Standardized Extract of Centella asiatica Prevents Fear Memory Deficit in 3xTg-AD Mice.
- Authors
Pairojana, Tanita; Phasuk, Sarayut; Tantisira, Mayuree H.; Liang, Kai-Chi; Roytrakul, Sittiruk; Pakaprot, Narawut; Chompoopong, Supin; Nudmamud-Thanoi, Sutisa; Ming, Yang; Liu, Ingrid Y.
- Abstract
ECa 233 is a standardized extract of Centella asiatica (CA), an herb widely used in traditional Chinese and Ayurvedic medicine. Previous studies reported that ECa 233 enhanced memory retention and synaptic plasticity in the hippocampus of healthy rats. Because of this, we became curious whether ECa 233 has a therapeutic effect on the fear memory deficit in the triple transgenic Alzheimer's disease (3xTg-AD) model mice. Fear memory is a crucial emotional memory for survival that is found to be impaired in patients with early-onset Alzheimer's disease (AD). In this study, we orally administered ECa 233 (doses: 10, 30, and 100 mg/kg) to 3xTg-AD mice, who were five months old, for 30 consecutive days. We found that ECa 233 prevented a cued fear memory deficit and enhanced hippocampal long-term potentiation (LTP) in 3xTg-AD mice. Subsequent proteomic and western blot analyses revealed increased expression levels of the molecules related to LTP induction and maintenance, including brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and its network proteins, and extracellular signal-regulated kinase 1 and 2 (ERK1 and 2) in the hippocampi and amygdala of 3xTg-AD mice after ECa 233 pre-treatment. Our results indicate that ECa 233 is a promising potential herbal standardized extract that could be used in preventing the fear memory deficit and synaptic dysfunction before the early onset of AD.
- Subjects
NEURAL transmission; ANIMAL behavior; BIOCHEMISTRY; STATISTICS; MEDICINAL plants; ALZHEIMER'S disease; HIPPOCAMPUS (Brain); ANIMAL experimentation; WESTERN immunoblotting; ONE-way analysis of variance; LIQUID chromatography; HUMAN locomotion; FEAR; NEUROTRANSMITTERS; CELL receptors; FISHER exact test; COMPARATIVE studies; PROTEOMICS; ELECTROPHYSIOLOGY; BIOINFORMATICS; PRE-tests &; post-tests; GENE expression; MEMORY disorders; DRUGS; RESEARCH funding; DESCRIPTIVE statistics; MASS spectrometry; REPEATED measures design; TRANSFERASES; PLANT extracts; BRAIN-derived neurotrophic factor; MITOGEN-activated protein kinases; AMYGDALOID body; DATA analysis software; DATA analysis; ANXIETY; MICE; VIDEO recording; PROMPTS (Psychology); PHOSPHORYLATION; PHARMACODYNAMICS
- Publication
American Journal of Chinese Medicine, 2023, Vol 51, Issue 2, p329
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X23500179