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- Title
The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer.
- Authors
Dianshui Sun; Xin Li; Maoqiang Ma; Jie Liu; Ying Xu; Lan Ye; Huaying Hou; Cuihong Wang; Xiaomei Li; Yuhua Jiang
- Abstract
Objective: The incidence of brain metastases greatly varies in patients with non-small-cell lung cancer, and molecular markers are considered to predict brain metastases. Therefore, this study sought to identify the predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in non-small-cell lung cancer. Methods: Patients who received a curable surgery for their lung cancer were screened according to our criteria. Formalin-fixed paraffin-embedded samples from the patients were examined for the expression of miRNA-328 and miRNA-378 using real-time polymerase chain reaction and the expression of N-cadherin, E-cadherin, vascular endothelial growth factor, protein kinase Cα and S100B were investigated using immunohistochemical staining. Results: In total, 86 patients were screened for this study and 23 patients were diagnosed with brain metastases during the follow-up period. Comparing patients with and without brain metastases, the expression of miRNA-328 and miRNA-378 in tumor tissues were significantly different (P=6.2 x 10-5 and P=2.8x 10-5, respectively). For the patients with brain metastases, the expression of miRNA-328 and miRNA-378 in tumor tissues compared with para-carcinoma tissues were also significantly different (P= 2.2 x 10-5 and P= 1.6 x 10-5, respectively). For patients with brain metastases, the association between miRNA-328 and protein kinase Cα was significant (r=0.591, P=0.003), but that between miRNA-378 and protein kinase Cα was not significant (r= 0.259, P= 0.232). Conclusions: The expression of miRNA-328 and miRNA-378 in tumor tissues can be used to predict brain metastases in patients with non-small-cell lung cancer. miRNA-328 might promote brain metastases by regulating the expression of protein kinase Ca. However, the mechanisms of miRNA-378 to promote brain metastases should be studied in the future.
- Publication
Japanese Journal of Clinical Oncology, 2015, Vol 45, Issue 5, p464
- ISSN
0368-2811
- Publication type
Article
- DOI
10.1093/jjco/hyv009