We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
In vitro and in vivo quantification of chloroprocaine release from an implantable device in a piglet postoperative pain model.
- Authors
Gregori, Simona De; Gregori, Manuela De; Bloise, Nora; Bugada, Dario; Molinaro, Mariadelfina; Filisetti, Claudia; Allegri, Massimo; Schatman, Michael E; Cobianchi, Lorenzo
- Abstract
Background: The pharmacokinetic properties and clinical advantages of the local anesthetic chloroprocaine are well known. Here, we studied the pharmacokinetic profile of a new hydrogel device loaded with chloroprocaine to investigate the potential advantages of this new strategy for postoperative pain (POP) relief. Materials and methods: We performed both in vitro and in vivo analyses by considering plasma samples of four piglets receiving slow-release chloroprocaine. To quantify chloroprocaine and its inactive metabolite 4-amino-2-chlorobenzoic acid (ACBA), a HPLC–tandem mass spectrometry (HPLC-MS/MS) analytical method was used. Serial blood samples were collected over 108 hours, according to the exposure time to the device. Results: Chloroprocaine was consistently found to be below the lower limit of quantification, even though a well-defined peak was observed in every chromatogram at an unexpected retention time. Concerning ACBA, we found detectable plasma concentrations between T0 and T12h, with a maximum plasma concentration (Cmax) observed 3 hours after the device application. In the in vitro analyses, the nanogel remained in contact with plasma at 37°C for 90 minutes, 3 hours, 1 day, and 7 days. Chloroprocaine Cmax was identified 1 day following exposure and Cmin after 7 days, respectively. Additionally, ACBA reached the Cmax following 7 days of exposure. Conclusion: A thorough review of the literature indicates that this is the first study analyzing both in vivo and in vitro pharmacokinetic profiles of a chloroprocaine hydrogel device and is considered as a pilot study on the feasibility of including this approach to the management of POP.
- Subjects
ARTIFICIAL implants; POSTOPERATIVE pain; BLOOD sampling; PIGLETS; LOCAL anesthetics
- Publication
Journal of Pain Research, 2018, Vol 11, p2837
- ISSN
1178-7090
- Publication type
Article
- DOI
10.2147/JPR.S180163