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- Title
Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat.
- Authors
Dige, Mahesh Shivanand; Kaushik, Rakesh; Kushwah, Bhavana; Singh, M. K.; Bhusan, Saket; Rout, P. K.
- Abstract
Insulin like growth factor (IGF 1) play an important role in the production and reproduction. One hundred and ninety two adult goats of Barbari and Jamunapari breed were taken for study. Data on growth traits were recorded to identify SNPs in the IGF-1gene by using High Resolution Melting (HRM) technique. Five fragments of IGF-1 were amplified length of amplicon were 105, 124, 150, 122 and 122 bp covering 5'UTR, 3'UTR, Exon 3, Exon 2 and 5'UTR regions, respectively and subsequently sequenced. The HRM analysis revealed no sequence variation among the samples of both breeds. Hence, the all five amplified fragments of IGF-1 gene showed monomorphic pattern and, no significant association could be detected with growth traits in Barbari and Jamunapari goats.
- Subjects
GOATS; GENETIC polymorphisms; CATTLE genetics; INSULIN; REPRODUCTION
- Publication
Indian Journal of Animal Research, 2020, Vol 54, Issue 11, p1451
- ISSN
0367-6722
- Publication type
Article
- DOI
10.18805/ijar.B-3893