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- Title
Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement.
- Authors
Suginami, Koh; Sato, Yukiyasu; Horie, Akihito; Matsumoto, Hisanori; Tani, Hirohiko; Mizumoto, Yasunari; Ono, Masanori; Matsuoka, Ayumi; Kyo, Satoru; Araki, Yoshihiko; Konishi, Ikuo; Fujiwara, Hiroshi
- Abstract
Problem We previously proposed that platelets promote re-epithelialization during menstruation. As cell movement is one of the important cell behaviors in the process of tissue remodeling, we examined the effects of platelets on endometrial epithelial cell invasion. Method of Study The platelets were isolated from healthy women. Using a human endometrial epithelial cell-derived immortalized cell line, EM-E6/E7/ hTERT cells, we examined the effects of platelets and platelet-derived condition media with or without microparticles on the morphological and invasive properties of EM-E6/E7/ hTERT cells. Results Platelets and microparticle-containing conditioned media inhibited Matrigel invasion by EM-E6/E7/ hTERT cells along with an increase in cortical ring formation, whereas microparticle-depleted conditioned media promoted their invasion without any significant changes of cortical ring formation. Conclusion These results support our previous proposal and newly suggest the dual roles of platelets: platelet-derived soluble factors that promote cell movement in the distant area, and microparticles that induce re-epithelialization by endometrial epithelial cells in the proximal area.
- Subjects
BLOOD platelets; ENDOMETRIUM; EPITHELIAL cells; CELL lines; CELL motility
- Publication
American Journal of Reproductive Immunology, 2017, Vol 77, Issue 4, pn/a
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12641