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- Title
Campylobacter occurrence and antimicrobial resistance profile in under five-year-old diarrheal children, backyard farm animals, and companion pets.
- Authors
Mulu, Wondemagegn; Joossens, Marie; Kibret, Mulugeta; Van den Abeele, Anne-Marie; Houf, Kurt
- Abstract
Campylobacteriosis disproportionately affects children under five in low-income countries. However, epidemiological and antimicrobial resistance (AMR) information at the children-animal interface is lacking. We hypothesized that Campylobacter is a major cause of enteritis in children in Ethiopia, and contact with animals is a potential source of transmission. The objective of the study was to determine Campylobacter occurrence and its AMR in children under five with diarrhea, backyard farm animals, and companion pets. Stool from 303 children and feces from 711 animals were sampled. Campylobacter was isolated through membrane filtration on modified charcoal cefoperazone deoxycholate agar plates under microaerobic incubation, and the technique showed to be feasible for use in regions lacking organized laboratories. Typical isolates were characterized with MALDI-TOF MS and multiplex PCR. Of 303 children, 20% (n = 59) were infected, with a higher proportion in the 6 to 11-month age group. Campylobacter occurred in 64% (n = 14) of dogs and 44% (n = 112) of poultry. Campylobacter jejuni was present in both a child and animal species in 15% (n = 23) of 149 households positive for Campylobacter. MICs using the gradient strip diffusion test of 128 isolates displayed resistance rates of 20% to ciprofloxacin and 11% to doxycycline. MICs of ciprofloxacin and doxycycline varied between C. coli and C. jejuni, with higher resistance in C. coli and poultry isolates. Campylobacter infection in children and its prevalent excretion from backyard poultry and dogs is a understudied concern. The co-occurrence of C. jejuni in animals and children suggest household-level transmission As resistance to ciprofloxacin and doxycycline was observed, therapy of severe campylobacteriosis should consider susceptibility testing. Findings from this study can support evidence-based diagnosis, antimicrobial treatment, and further investigations on the spread of AMR mechanisms for informed One Health intervention. Author summary: Diarrheal diseases are the second-leading cause of death in children under five years, and Campylobacter is a major global causative agent. However, little attention is given to the accurate isolation, characterization, and AMR assessment of Campylobacter in sub-Saharan Africa. This region has the highest burden of diarrhea but a lack of organized laboratories. This study investigated the presence and AMR of Campylobacter in children and animals using methods with minimal technical challenge. We collected stool samples from 303 children at health centers and 711 animal feces samples from the children's homes in the vicinity of Bahir Dar, Ethiopia. Campylobacter were isolated using membrane filtration-culture. Presumptive isolates were characterized with molecular techniques in Belgium. Membrane filtration culture with a traditional microaerobic incubation system was successful in isolating viable Campylobacter from children with diarrhea and animals. Campylobacter is highly prevalent in children, backyard poultry, and dogs and has shown resistance to ciprofloxacin and doxycycline. The key findings of this study clearly show that Campylobacter infection in children and its occurrence in animals associated with children is a major public health problem. These findings are supportive of clinical decision-making and treatment. Findings from this study are also relevant as a validation of applying the membrane filtration-isolation technique for Campylobacter screening in low-income countries.
- Subjects
SUB-Saharan Africa; ETHIOPIA; CAMPYLOBACTER; DRUG resistance in microorganisms; DOMESTIC animals; CAMPYLOBACTER jejuni; INSTITUTIONAL care of children; DOG bites
- Publication
PLoS Neglected Tropical Diseases, 2024, Vol 18, Issue 6, p1
- ISSN
1935-2727
- Publication type
Article
- DOI
10.1371/journal.pntd.0012241