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- Title
Assessment of mitochondrial DNA viability ratio in day-4 biopsied embryos as an add-in to select euploid embryos for single embryo transfer.
- Authors
Metwalley, Ahmad; Hellani, Ali; Abdelrazek, Azza A.; El-Damen, Ahmed; Al Dawood, Ahmed; Brasha, Nabeel; Esteves, Sandro C.; El Hamshary, Manal; Khamiss, Omaima
- Abstract
Summary: The aim of this study was to assess mitochondrial DNA analysis as a predictor of the pregnancy potential of biopsied preimplantation embryos. The study included 78 blastomeres biopsied from day 4 cleavage stage euploid embryos. The embryo karyotype was confirmed by 24-chromosome preimplantation genetic testing for aneuploidies using the Illumina Next-Generation Sequencing (NGS) system. Mitochondria viability ratios (mtV) were determined from BAM files subjected to the web-based genome-analysis tool Galaxy. From this cohort of patients, 30.4% of patients (n = 34) failed to establish pregnancy. The mean mtV ratio [mean = 1.51 ± 1.25–1.77 (95% CI)] for this group was significantly (P < 0.01) lower compared with the embryo population that resulted in established pregnancies [mean = 2.5 ± 1.82–2.68 (95% CI)]. mtV multiple of mean (MoM) values were similarly significantly (P < 0.01) lower in blastocysts failing to establish pregnancy. At a 0.5 MoM cut-off, the sensitivity of mtV quantitation was 35.3% and specificity was 78.2%. The positive predictive value for an mtV value > 0.5 MoM was 41.4%. This study demonstrates the clinical utility of preimplantation quantification of viable mitochondrial DNA in biopsied blastomeres as a prognosticator of pregnancy potential.
- Subjects
MITOCHONDRIAL DNA; EMBRYO transfer; EMBRYOS; BLASTOMERES; DNA analysis; MULTIPLE pregnancy
- Publication
Zygote, 2022, Vol 30, Issue 6, p790
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199422000260