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- Title
The Morphology and the Biological Control of Cryphonectria parasitica.
- Authors
PUIA, Carmen Emilia; GRIGORESCU, Daniela Andreea; MICLEA, Raluca Vasilica
- Abstract
Cryphonectria parasitica (Murr.) Bar [syn. Endothia parasitica (Murr. And.] (anamorf: Endothiella sp.) is the causal agent of chestnut bark disease or chestnut blight, disease which produced great damages throughout the world, for example, in Europe, the European chestnut tree (Castanea saftva(P.) Mill) was heavily affected. Environmental concerns have focused attention on natural forms of disease control as an effective alternative to chemical pesticides. In the chestnut blight fungus, Cryphonectria parasitica deals with a natural form of biological control in which the virulence of a fungal pathogen is attenuated by an endogenous viral RNA genetic element- the hypovirulent strain. In our researches we picked samples of chestnut bark from different areas in Maramures county. We've isolated the fungus on PDA medium and we've studied the morphological characteristics of the usual virulent strain and we looked for a possible hypovirulent strain in order to study its capacity for biological control. The fungus develops in the bark and in cambium where forms a yellowish or brownish stroma and produces both conidia and ascospores. The pycnidia stromata break through the lenticels producing conidia and later in the same stroma develop the perithecia which produce ascospores. Both strains of the fungus were found in the research area. The hypovirulent strain had a slower development, showed no sporulation and pigmentation "white cultural strain" and was tested in vitro for the capacity to convert the virulent isolates by dual culture tests.
- Subjects
ENDOTHIA parasitica; ASCOMYCETES; AGRICULTURAL chemicals; NUCLEIC acids; PLANT spores
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Agriculture, 2012, Vol 69, Issue 1, p353
- ISSN
1843-5246
- Publication type
Article
- DOI
10.15835/buasvmcn-agr:8720