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- Title
类芽孢杆菌 CH2 的筛选、酶活性及全基因组分析.
- Authors
陈芝娟; 王虹军; 田 星; 张 根
- Abstract
Mangrove soil is a reliable source for screening cellulose-degrading bacteria due to the high diversity of microbes. To effectively utilize crop straw resources, a cellulolytic bacterium, Paenibacillus silvae strain CH2 was isolated from mangrove soil. We determined the carboxymethyl cellulose (CMC) and filter paper assay (FPA) activities of CH2 at different incubation times, NaCl concentrations, pH and temperatures, estimated the degradation efficiencies of rice and maize straw by CH2, sequenced and analyzed the whole genome of CH2. The results showed that along with the increases of incubation time, NaCl concentration, pH and temperature, the CMC and FPA activities increased first and then decreased . The highest CMC and FPA activities were observed at incubation time of 72-84 h, NaCl concentration of 6.0 g·L -1, pH of 7 and temperature of 36 . Degradation of straw assays revealed that CH2 could effectively degrade rice and maize straw. At 0 g · L-1 NaCl (the control), the 10-day degradation rates of rice and maize straw were 30.4% and 47.0%, respectively. In the presence of 15 g · L-1 NaCl, the degradation rates were not significantly different from the control, indicating that CH2 had a high tolerance to salts. The whole genome of P. silvae CH2 was 6797325 bp, containing 6312 coding genes. P. silvae CH2 contained multiple genes encoding cellulose and hemicellulose degrading enzymes. These enzymes mainly belonged to the GH family, including endo-1,4-β-xylanase, Xylan 1,4-β-xylosidase, β-glucosidase, and endoglucanase. The results indicated that the bacterium had the potential to be used in crop straw degradation.
- Subjects
MANGROVE forests; LIGNOCELLULOSE; STRAW; GENOMES; SALT
- Publication
Yingyong Shengtai Xuebao, 2023, Vol 34, Issue 12, p3404
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202312.030