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- Title
In situ cryo-electron tomography: a new method to elucidate cytoplasmic zoning at the molecular level.
- Authors
Chen, Lin; Fukata, Yuko; Murata, Kazuyoshi
- Abstract
Cryo-electron microscopy was developed as a powerful tool for imaging biological specimens in near-native conditions. Nowadays, advances in technology, equipment and computations make it possible to obtain structures of biomolecules with near-atomic resolution. Furthermore, cryo-electron tomography combined with continuous specimen tilting allows structural analysis of heterogeneous biological specimens. In particular, when combined with a cryo-focused ion beam scanning electron microscope, it becomes possible to directly analyse the structure of the biomolecules within cells, a process known as in situ cryo-electron tomography. This technique has the potential to visualize cytoplasmic zoning, involving liquid–liquid phase separation, caused by biomolecular networks in aqueous solutions, which has been the subject of recent debate. Here, we review advances in structural studies of biomolecules to study cytoplasmic zoning by in situ cryo-electron tomography.
- Subjects
ZONE melting; TOMOGRAPHY; SCANNING electron microscopes; BIOLOGICAL specimen analysis; ELECTRON beams; ION beams; FOCUSED ion beams
- Publication
Journal of Biochemistry, 2024, Vol 175, Issue 2, p187
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/jb/mvad102