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- Title
vAL-1, a novel polysaccharide lyase encoded by chlorovirus CVK2
- Authors
Sugimoto, Ichiro; Onimatsu, Hideki; Fujie, Makoto; Usami, Shoji; Yamada, Takashi
- Abstract
Cell wall materials isolated from Chlorella cells were degraded by the polysaccharide-degrading enzyme vAL-1 encoded by chlorovirus CVK2. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analyses of the degradation products (oligosaccharides) revealed major oligosaccharides contain unsaturated GlcA at the reducing terminus, and a side chain attached at C2 or C3 of GlcA(C4&z.dbnd6;C5), which mainly consisted of Ara, GlcNAc and Gal. The results indicated that vAL-1 is a novel polysaccharide lyase, cleaving chains of β- or α-1,4-linked GlcAs. The unique structures of Chlorella cell wall were also revealed. Studies on the complicated structures of naturally occurring polysaccharides will be greatly facilitated by using vAL-1 as a tool in structural analysis.
- Subjects
POLYSACCHARIDES; ENZYMES; CHLORELLA; SPECTROMETRY
- Publication
FEBS Letters, 2004, Vol 559, Issue 1-3, p51
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(04)00022-5