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- Title
Dynamic analysis of STAT6 signalling in living cells
- Authors
Nelson, Glyn; Wilde, Geraint J.C.; Spiller, David G.; Sullivan, Elaine; Unitt, John F.; White, Michael R.H.
- Abstract
Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP–STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-κB pathway through TNFα treatment, or expression of p65–EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-κB activation.
- Subjects
HELA cells; NF-kappa B
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p188
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03672-4