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- Title
Effect of annexin I on insulin secretion through surface binding sites in rat pancreatic islets
- Authors
Hong, Shin-Hee; Won, Jong Hak; Yoo, Seung-Ah; Auh, Chung-Kyoon; Park, Young Min
- Abstract
This study investigates the effect of extracellular annexin I (Anx I) on regulating insulin secretion in isolated rat pancreatic islets. Results show that Anx I stimulates insulin release in pancreatic islets regardless of the presence or absence of extracellular Ca2+. In particular, confocal microscopy shows that Anx I binds to the surface of islet cells in the absence of extracellular Ca2+. However, insulin secretion through Anx I significantly decreases in trypsin-treated islets. Likewise, there is minimal binding of Anx I to the surface of trypsin-treated islets. Anti-Anx I polyclonal antibody also inhibits the stimulating effect of Anx I on insulin secretion. These results indicate that Anx I is capable of binding to the cell surface receptor, in order to regulate the stimulation of insulin release in rat pancreatic islets.
- Subjects
ANNEXINS; INSULIN
- Publication
FEBS Letters, 2002, Vol 532, Issue 1/2, p17
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)03613-X