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- Title
Cell-line specific chromatin acetylation at the Sox10–Pax3 enhancer site modulates the RET proto-oncogene expression
- Authors
Puppo, Francesca; Griseri, Paola; Fanelli, Mirco; Schena, Francesca; Romeo, Giovanni; Pelicci, PierGiuseppe; Ceccherini, Isabella; Ravazzolo, Roberto; Patrone, Giovanna
- Abstract
The RET gene is expressed with high tissue and stage specificity during development. Understanding its transcriptional regulation might provide new clues to clarify developmental mechanisms. Here we show that the histone deacetylase inhibitor sodium butyrate (NaB) increases RET transcription in cells displaying low levels of its mRNA, while it has no effect in cells expressing at high levels. Chromatin immunoprecipitation (ChIP) experiments showed increased histone acetylation within the same region, in particular the Sox10–Pax3 enhancer site, due to NaB. Accordingly, ChIP showed different acetylation levels at the Sox10–Pax3 site associated with cell-line specific RET transcription rates. Concluding, chromatin acetylation targeted to functional sequences in the RET regulatory region may control its transcription.
- Subjects
CHROMATIN; GENETIC transcription; HISTONES; ACETYLATION
- Publication
FEBS Letters, 2002, Vol 523, Issue 1-3, p123
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(02)02957-5