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- Title
Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles.
- Authors
Härmä, Harri; Toivonen, Juha; Soini, Juhani T.; Hänninen, Pekka; Parak, Wolfgang J.
- Abstract
Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
- Subjects
FLUORESCENCE; NANOPARTICLES; SEMICONDUCTORS; LUMINESCENCE; C-reactive protein
- Publication
Sensors (14248220), 2011, Vol 11, Issue 12, p11335
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s111211335