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- Title
A Salmonella virulence protein that inhibits cellular trafficking.
- Authors
Kei-ichi Uchiya; Barbieri, M. Alejandro; Funato, Kouichi; Shah, Ankur H.; Stahl, Philip D.; Groisman, Eduardo A.
- Abstract
Salmonella enterica requires a type III secretion system, designated Spi/Ssa, to survive and proliferate within macrophages. The Spi/Ssa system is encoded within the SPI-2 pathogenicity island and appears to function intracellularly. Here, we establish that the SPI-2-encoded SpiC protein is exported by the Spi/Ssa type In secretion system into the host cell cytosol where it interferes with intracellular trafficking. In J774 macrophages, wild-type Salmonella inhibited fusion of Salmonella-containing phagosomes with lysosomes and endosomes, and interfered with trafficking of vesicles devoid of the microorganism. These inhibitory activities required living Salmonella and a functional spiC gene. Purified SpiC protein inhibited endosome-endosome Fusion in vitro. A Sindbis virus expressing the SpiC protein interfered with normal trafficking of the transferrin receptor in vivo. A spiC mutant was attenuated for virulence, suggesting that the ability to interfere with intracellular trafficking is essential for Salmonella pathogenesis.
- Subjects
SALMONELLA enteritidis; PROTEINS; LYSOSOMES; MICROORGANISMS; CELLS; CYTOSOL
- Publication
EMBO Journal, 1999, Vol 18, Issue 14, p3924
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/18.14.3924