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- Title
Developed <sub>7</sub>M Haemolytic Plaque-forming Cells in Chickens.
- Authors
Shand, F.L.; Ivanyi, J.
- Abstract
Incorporation of rabbit anti-μ serum into agarose gels inhibited direct anti-SRBC PFCs using chicken complement, but developed equal numbers of indirect PFCs binding guinea-pig complement. The majority of attempts to demonstrate direct PFCs against HSA were unsuccessful, although low numbers of barely discernible PFCs were obtained by using HSA-SRBC sensitized with carbodiimide at room temperature; indirect PFCs were developed, however, by the anti-p serum. During the primary response, developed anti-HSA PFCs could not be assigned to IgM or IgG classes using reduction and alkylation. Concanavalin A completely inhibited direct anti-SRBC and Sill PFCs, although developed γM PFCs were only inhibited partially. The identity of direct and anti-: developed PFCs against Sill was demonstrated by the replica technique. None of the approaches applied provided any support for the existence of structural heterogeneity of IgM within the PFC population.
- Subjects
HEMOLYTIC plaque technique; BLOOD plasma; COLLOIDS; GELATION; IMMUNOGLOBULIN G; CHEMICAL reactions
- Publication
Immunology, 1973, Vol 24, Issue 4, p759
- ISSN
0019-2805
- Publication type
Article