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- Title
Amylin Gene Sequence of the Amyloidogenic Region in the Velvet Monkey (Chlorocebus aethiops).
- Authors
Gray, Stanton B.; Howard, Timothy; Hawkins, Gregory; Diallo, Abdoulaye; Wagner, Janice
- Abstract
We are in the process of developing the vervet monkey (Chlorocebus aethiops) as a model of obesity and Type 2 diabetes. In one step among many towards; that goal, we report DNA sequence data from the amyloidogenic region of the amylin gene. Amino acid sequence is subsequently inferred and compared with human and macaque monkey sequence. Amino acid residues 20-29 are thought to play a role in Type 2 diabetes-associated fibril formation of Islet Amyloid Polypeptide (IAPP) and are thus referred to as the amyloidogenic region (AR). IAPP is expressed in pancreatic islets of all mammalian species, but the propensity to form amyloid is related to species specific differences pertaining to proline substitutions within the AR. We sequenced 24 presumably unrelated vervets and found that the inferred residue 20-29 amino acid sequence is identical to macaque monkeys in that it differs from the human sequence at residue 25 (Threonine instead of Alanine). Further, we found no praline residues in the AR, similar to humans and macaque monkeys. Therefore, Type 2 diabetic velvets should show islet pathology involving amyloid deposition, similar or identical to diabetic humans and macaques. In addition, we discovered 2 Single Nucleotide Polymorphisms (SNPs) in the coding region of the amylin gene adjacent to the AR. The significance of these SNPs has yet to be determined, but it is interesting to note that these SNPs are close to established SNPs in the human sequence.
- Subjects
AMYLIN; LABORATORY monkeys; OBESITY; TYPE 2 diabetes; NUCLEOTIDE sequence; PEPTIDES; GENETIC polymorphisms
- Publication
Diabetes, 2007, Vol 56, pA691
- ISSN
0012-1797
- Publication type
Article