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- Title
Separation and Purification of Hydroxyl-α-Sanshool from Zanthoxylum armatum DC. by Silica Gel Column Chromatography.
- Authors
Cheng, Jinxi; Hou, Xiaoyan; Cui, Qiang; Shen, Guanghui; Li, Shanshan; Luo, Qingying; Zhou, Man; Chen, Hong; Zhang, Zhiqing
- Abstract
Hydroxyl-α-sanshool is the main alkylamide produced by Zanthoxylum armatum DC., and it is responsible for numbness after consuming Z. armatum-flavored dishes or food products. The present study deals with the isolation, enrichment, and purification of hydroxyl-α-sanshool. The results indicated that the powder of Z. armatum was extracted with 70% ethanol and then filtrated; the supernatant was concentrated to get pasty residue. Petroleum ether (60–90 °C) and ethyl acetate at a 3:2 ratio, with an Rf value of 0.23, were chosen as the eluent. Petroleum ether extract (PEE) and ethyl acetate–petroleum ether extract (E-PEE) were used as the suitable enriched method. Afterward, the PEE and E-PEE were loaded onto silica gel for silica gel column chromatography. Preliminary identification was carried out by TLC and UV. The fractions containing mainly hydroxyl-α-sanshool were pooled and dried by rotary evaporation. Lastly, all of the samples were determined by HPLC. The yield and recovery rates of hydroxyl-α-sanshool in the p-E-PEE were 12.42% and 121.65%, respectively, and the purity was 98.34%. Additionally, compared with E-PEE, the purity of hydroxyl-α-sanshool in the purification of E-PEE (p-E-PEE) increased by 88.30%. In summary, this study provides a simple, rapid, economical, and effective approach to the separation of high-purity hydroxyl-α-sanshool.
- Subjects
NORMAL-phase chromatography; SILICA gel; COLUMN chromatography; ZANTHOXYLUM; THIN layer chromatography; ETHER (Anesthetic); ETHYL acetate
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 4, p3156
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24043156