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- Title
Biological Evaluation and Transcriptomic Analysis of Corylin as an Inhibitor of Osteoclast Differentiation.
- Authors
Yu, Anna Xiao-Dan; Xiao, Jian; Zhao, Shi-Zheng; Kong, Xiang-Peng; Kwan, Kenneth Kin-Leung; Zheng, Brody Zhong-Yu; Wu, Kevin Qi-Yun; Dong, Tina Ting-Xia; Tsim, Karl Wah-Keung; Kim, Jung Eun
- Abstract
Corylin, a flavonoid isolated from the fruit of Psoralea corylifolia, has an osteogenic effect on osteoblasts in vitro and bone micromass ex vivo. However, the effect and mechanism of corylin in regulating osteoclastogenesis remain unknown. By using murine bone marrow macrophages as the osteoclast precursor, corylin was found to inhibit the receptor activator of nuclear factor (NF) κB ligand (RANKL)-induced osteoclast differentiation via down-regulating osteoclastic marker genes. In parallel, F-actin formation and osteoclast migration were diminished in corylin-treated cultured osteoclasts, and subsequently the expressions of osteoclastic proteins were suppressed: the suppression of protein expression was further illustrated by transcriptomic analysis. Furthermore, corylin inhibited the nuclear translocation of p65, giving rise to a restraint in osteoclastic differentiation through the attenuation of transcription factors nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and nuclear factor of activated T cells c1 (NFATc1). There was no obvious change in apoptosis when the RANKL-induce osteoclasts were cultured in the presence of corylin. The finding supports the potential development of corylin as an osteoclast inhibitor against osteoporosis.
- Subjects
OSTEOCLASTOGENESIS; BONE marrow; B cells; OSTEOCLASTS; TRANSCRIPTION factors; GENES; T cells
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 7, p3540
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22073540