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- Title
Clinicopathologic Analysis of Cathepsin B as a Prognostic Marker of Thyroid Cancer.
- Authors
Kim, Eun-Kyung; Song, Min-Jeong; Jang, Ho Hee; Chung, Yoo Seung
- Abstract
Thyroid cancer incidence has increased worldwide; however, investigations of thyroid cancer-related factors as potential prognosis markers remain insufficient. Secreted proteins from the cancer secretome are regulators of several molecular mechanisms and are, thereby, ideal candidates for potential markers. We aimed to identify a specific factor for thyroid cancer by analyzing the secretome from normal thyroid cells, papillary thyroid cancer (PTC) cells, and anaplastic thyroid cancer cells using mass spectrometry (MS). Cathepsin B (CTSB) showed highest expression in PTC cells compared to other cell lines, and CTSB levels in tumor samples were higher than that seen in normal tissue. Further, among thyroid cancer patients, increased CTSB expression was related to higher risk of lymph node metastasis (LNM) and advanced N stage. Overexpression of CTSB in thyroid cancer cell lines activated cell migration by increasing the expression of vimentin and Snail, while its siRNA-mediated silencing inhibited cell migration by decreasing vimentin and Snail expression. Mechanistically, CTSB-associated enhanced cell migration and upregulation of vimentin and Snail occurred via increased phosphorylation of p38. As our results suggest that elevated CTSB in thyroid cancer induces the expression of metastatic proteins and thereby leads to LNM, CTSB may be a good and clinically relevant prognostic marker.
- Subjects
CATHEPSIN B; THYROID cancer; ANAPLASTIC thyroid cancer; TUMOR markers; CELL migration
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 24, p9537
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21249537