We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Wild Soybean Oxalyl-CoA Synthetase Degrades Oxalate and Affects the Tolerance to Cadmium and Aluminum Stresses.
- Authors
Xian, Peiqi; Cai, Zhandong; Cheng, Yanbo; Lin, Rongbin; Lian, Tengxiang; Ma, Qibin; Nian, Hai
- Abstract
Acyl activating enzyme 3 (AAE3) was identified as being involved in the acetylation pathway of oxalate degradation, which regulates the responses to biotic and abiotic stresses in various higher plants. Here, we investigated the role of Glycine sojaAAE3 (GsAAE3) in Cadmium (Cd) and Aluminum (Al) tolerances. The recombinant GsAAE3 protein showed high activity toward oxalate, with a Km of 105.10 ± 12.30 μM and Vmax of 12.64 ± 0.34 μmol min−1 mg−1 protein, suggesting that it functions as an oxalyl–CoA synthetase. The expression of a GsAAE3–green fluorescent protein (GFP) fusion protein in tobacco leaves did not reveal a specific subcellular localization pattern of GsAAE3. An analysis of the GsAAE3 expression pattern revealed an increase in GsAAE3 expression in response to Cd and Al stresses, and it is mainly expressed in root tips. Furthermore, oxalate accumulation induced by Cd and Al contributes to the inhibition of root growth in wild soybean. Importantly, GsAAE3 overexpression increases Cd and Al tolerances in A. thaliana and soybean hairy roots, which is associated with a decrease in oxalate accumulation. Taken together, our data provide evidence that the GsAAE3-encoded protein plays an important role in coping with Cd and Al stresses.
- Subjects
FLUORESCENT proteins; CADMIUM; CHIMERIC proteins; RECOMBINANT proteins; ALUMINUM; RUMEX; SOYBEAN; CADMIUM poisoning
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 22, p8869
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21228869