We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins.
- Authors
Pérez-Dorado, Inmaculada; Bortolotti, Ana; Cortez, Néstor; Hermoso, Juan A.
- Abstract
Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50's loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as "short-chain" with the firmicutes flavodoxins and the "long-chain" with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.
- Subjects
RHODOBACTER capsulatus; CRYSTAL structure; FLAVODOXIN; PROTOTYPES; NITROGEN fixation; PHYLOGENY; CLUSTER analysis (Statistics); NITROGENASES; ELECTROSTATICS
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 1, p1152
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms14011152