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- Title
A Chinese herbal formula 'Gan-Lu-Yin' suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.
- Authors
Yi-Chung Chien; Ming-Jyh Sheu; Chieh-Hsi Wu; Wen-Hsin Lin; Ying-Yi Chen; Po-Liang Cheng; Hsu-Chen Cheng
- Abstract
Background: This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury. Methods: VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining. Results: Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis. Conclusions: These observations provide a mechanism of GLY in attenuating cell migration, thus as a potentialintervention for restenosis.
- Publication
BMC Complementary & Alternative Medicine, 2012, Vol 12, Issue 1, p137
- ISSN
1472-6882
- Publication type
Journal Article
- DOI
10.1186/1472-6882-12-137