We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Expression of Recombinant Proteins in Pichia Pastoris.
- Authors
Pingzuo Li; Anukanth Anumanthan; Xiu-Gong Gao; Kuppusamy Ilangovan; Vincent Suzara; Nejat Düzgüneş; V. Renugopalakrishnan
- Abstract
Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
- Subjects
RECOMBINANT proteins; PICHIA pastoris; GENOMES; GLYCOSYLATION
- Publication
Applied Biochemistry & Biotechnology, 2007, Vol 142, Issue 2, p105
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-007-0003-x