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- Title
Kinetic mechanisms by which nickel alters the calcium (Ca<sup>2+</sup>) transport in intact rat liver.
- Authors
Utsunomiya, Karina Sayuri; da Silva, Lucas Jonatas; Iwamoto, Juliana; Constantin, Rodrigo Polimeni; Gilglioni, Eduardo Hideo; Constantin, Jorgete; Bracht, Adelar; Elferink, Ronald Petrus Johannes Oude; Ishii-Iwamoto, Emy Luiza
- Abstract
In the present work, the multiple-indicator dilution (MID) technique was used to investigate the kinetic mechanisms by which nickel (Ni2+) affects the calcium (Ca2+) transport in intact rat liver. 45Ca2+ and extra- and intracellular space indicators were injected in livers perfused with 1 mM Ni2+, and the outflow profiles were analyzed by a mathematical model. For comparative purposes, the effects of norepinephrine were measured. The influence of Ni2+ on the cytosolic Ca2+ concentration ([Ca2+]c) in human hepatoma Huh7 cells and on liver glycogen catabolism, a biological response sensitive to cellular Ca2+, was also evaluated. The estimated transfer coefficients of 45Ca2+ transport indicated two mechanisms by which Ni2+ increases the [Ca2+]c in liver under steady-state conditions: (1) an increase in the net efflux of Ca2+ from intracellular Ca2+ stores due to a stimulus of Ca2+ efflux to the cytosolic space along with a diminution of Ca2+ re-entry into the cellular Ca2+ stores; (2) a decrease in Ca2+ efflux from the cytosolic space to vascular space, minimizing Ca2+ loss. Glycogen catabolism activated by Ni2+ was transient contrasting with the sustained activation induced by norepinephrine. Ni2+ caused a partial reduction in the norepinephrine-induced stimulation in the [Ca2+]c in Huh7 cells. Our data revealed that the kinetic parameters of Ca2+ transport modified by Ni2+ in intact liver are similar to those modified by norepinephrine in its first minutes of action, but the membrane receptors or Ca2+ transporters affected by Ni2+ seem to be distinct from those known to be modulated by norepinephrine.
- Subjects
LIVER; LIVER cells; NICKEL; RATS; CATABOLISM; CALCIUM; CALCIUM channels
- Publication
Journal of Biological Inorganic Chemistry (JBIC), 2021, Vol 26, Issue 6, p641
- ISSN
0949-8257
- Publication type
Article
- DOI
10.1007/s00775-021-01883-7