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- Title
Photoacoustic recovery after photothermal bleaching in living cells.
- Authors
Chiye Li; Chi Zhang; Liang Gao; Garcia-Uribe, Alejandro; Wang, Lihong V.
- Abstract
We present an innovative method, photoacoustic recovery after photothermal bleaching (PRAP), for studying particle dynamics at micron scale via photoacoustic imaging. As an intuitive way to visualize and quantify dynamic processes, PRAP is demonstrated first in a simple phantom study and then in a more complex measurement involving live cells. Compared with the conventional fluorescence-based approach, PRAP provides high signal-to-noise ratio (SNR) imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic processes inside a cell.
- Subjects
PHOTOACOUSTIC spectroscopy; PHOTOTHERMAL effect; ELECTRIC bleaching; NANOPARTICLES; DYNAMICS; DIFFUSION kinetics
- Publication
Journal of Biomedical Optics, 2013, Vol 18, Issue 10, p1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.18.10.106004