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- Title
Metabolism of sulphobromophthalein I: positional isomers of sulphobromophthalein monoglutathione conjugate.
- Authors
Sano, Kazumi; Totsuka, Yukari; Ikegami, Yoji; Uesugi, Takashi
- Abstract
Three positional isomers of sulphobromophthalein glutathione monoconjugate (BSP-mGSH) were detected using a paired-ion HPLC method that employs triethylamine phosphate (TEA-H3PO4) as a pairing agent. To confirm that these compounds were glutathione (GSH) conjugates, sulphobromophthalein (BSP) was incubated with a four-fold volume of GSH under alkaline ammonium hydroxide. At least 6 metabolites (3 di-GSH conjugates and 3 isomers of mono-GSH conjugates) were produced under these conditions. The three mono-GSH conjugates were each purified and identified as compounds with a molecular weight of 1020 according to FAB mass spectrometry results. Positional isomers of BSP-GSH were provisionally distinguished via the addition of the symbols α, β and δ to the end of each abbreviation, to reflect the amount of isomers present. Thus, the isomer present in the largest quantity was termed BSP-mGSH(α), the second most abundant isomer was termed BSP-mGSH(β) and the third was termed BSP-mGSH(δ). Interestingly, a species difference was recognized in that rat cytosol GSH S-transferase (GST) primarily produced BSP-mGSH(α), whereas guinea-pig cytosol generated BSP-mGSH(δ), BSP-mGSH(α) and BSP-mGSH(β) equally and rabbit cytosol mainly produced BSP-mGSH(β).
- Publication
Journal of Pharmacy & Pharmacology, 2001, Vol 53, Issue 7, p1015
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1211/0022357011776261