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- Title
ANTIMIKROBIÁLNY ÚČINOK VYBRANÝCH ZMESÍ PRÍRODNÝCH LÁTOK.
- Authors
Katarína, KAŠLÍKOVÁ; Patrik, BEŇOVIČ; Zdenka, KRAJČOVIČOVÁ; Vladimír, MELUŠ
- Abstract
Background: The antimicrobial properties of natural compounds have been the subject of research interest in the last decade, also in terms of studying the mechanism of their action. Objective: The objective of our study was to verify the degree of antibacterial efficacy of selected mixtures of natural antibacterial agens and to compare it to commonly used laboratory disinfectants, vinegar, and selected antibiotics. Material and methods: To determine the antimicrobial effect, we tested a tincture of sage (Salvia officinalis), Australian tea tree oil (Melaluca alternifolia), and garlic (Allium sativum). For comparison, we chose disinfectants based on alcohol, chlorine, and a quaternary ammonium compound in combination with alcohols, kitchen vinegar, as well as antibiotics, which we chose according to the recommendations of the EUCAST reference center for individual strains of bacteria. All antibacterial agents were tested using three different bacterial strains: Staphylococcus aureus (CCM 3953), Klebsiella pneumoniae (a bacterial strain isolated from the hospital environment) and Pseudomonas aeruginosa (CCM 3955). To test the antibacterial effect of selected mixtures of natural compounds and disinfectants, we used the standard qualitative biological suspension method, which is used to test the effectiveness of disinfectants. When testing the susceptibility of bacteria to antibiotics, we chose the disk diffusion method. Results: Testing showed antibacterial properties in all tested mixtures of natural antibacterial substances, but most notably in the case of sage extract, which showed an inhibitory antimicrobial effect even with longer exposure times (more than 30 minutes) and which was comparable to the effect of kitchen vinegar. The results support the hypothesis of the presence of antibacterial effects of test substances, which could in certain circumstances be a choice for current antibiotics and disinfectants. Conclusion: Nowadays, when there are increasing rates of resistance of microorganisms to antibiotics and disinfectants can be natural substances valuable alternative to use as antimicrobial agents, "posledná veta je nezrozumiteľná - utajené "ČO" môže byť natural substances valuable alternative: "resistance of microorganisms to antibiotics and disinfectants SOMETHING can be natural substances valuable alternative to use as antimicrobial agents.".
- Publication
Zdravotnicke listy, 2021, Vol 9, Issue 2, p79
- ISSN
1339-3022
- Publication type
Article