We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
榅桲果实CAD基因的克隆、序列分析及表达.
- Authors
车玉红; 杨波; 郭春苗; 木巴热克·阿尤普; 吴津蓉; 杜鹃
- Abstract
【Objective】 To investigate the relationship between the lignization and CAD genes in Quince Frui.【Method】Based on the cDNA sequence of cinnamyl alcohol dehydrogenase (CAD) gene reported by GenBank, PCR primers were designed by primer premier 5.0. The total RNA was extracted and the cDNA was synthesized by reverse transcription. The CAD gene fragment was successfully amplified by RT-PCR and cloned into pMD 18-T vector. Through the homologous sequence alignment of DNAStar software, clustalx combined with mega 4.1 software, the phylogenetic tree was built and the physicochemical properties of proteins were analyzed by protparam online program, and the secondary structure was predicted by protean program of dnastar. Finally, the accumulation of lignin in pulp during pulp development was identified by resorcinol staining, and the expression of CAD gene during development was detected by RT-qPCR. 【Result】 The results showed that the open reading frame (ORF) sequence of quince CAD gene was 1,071 bp, encoding 356 amino acids, and the homology with other species was as high as 96%, which was close to apple in evolutionary relationship. During the six stages of fruit development, the accumulation of lignin decreased gradually, while the expression of CAD gene was closely related to the degree of lignin fruit, and showed a trend of decreasing step by step with the slow synthesis.【Conclusion】CAD gene is the key gene to regulate the lignification of Quince Fruit.
- Publication
Xinjiang Agricultural Sciences, 2020, Vol 57, Issue 5, p814
- ISSN
1001-4330
- Publication type
Article
- DOI
10.6048/j.issn.1001-4330.2020.05.004