We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Degradation of Aflatoxin B 1 in Moldy Maize by Pseudomonas aeruginosa and Safety Evaluation of the Degradation Products.
- Authors
Xu, Yanhua; Zhao, Renyong; Liu, Chenxi
- Abstract
Aflatoxin B1 (AFB1) is the most harmful mycotoxin commonly found in food and feed. Pollution from AFB1 causes serious economic and health issues worldwide because it causes strong mutagenicity and carcinogenicity in humans and animals. In this study, Pseudomonas aeruginosa was used to degrade AFB1 in moldy maize, and the safety of this biological method was investigated using genotoxicity and cytotoxicity tests. Using response surface methodology, we established the optimal conditions for degrading AFB1 by the fermentation supernatant of P. aeruginosa. Under these conditions, the degradation rate of AFB1 reached 99.67%. Furthermore, the Ames mutagenicity test showed that AFB1 treated with P. aeruginosa fermentation supernatant for 72 h was not mutagenic. CCK-8 cell assay showed that AFB1 cytotoxicity was significantly reduced after degradation. Overall, our findings show that the fermentation supernatant of P. aeruginosa may be a good candidate for biodegradation of AFB1.
- Subjects
AMES (Iowa); PSEUDOMONAS aeruginosa; AFLATOXINS; CORN; MUTAGENICITY testing; RESPONSE surfaces (Statistics); AMES test
- Publication
Foods, 2023, Vol 12, Issue 6, p1217
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods12061217