We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
IMMUNOHISTOCHEMICAL MARKERS USEFUL IN DIFFERENTIATING THYROID NODULES.
- Authors
Stan, V.; Cornianu, M.; Dema, A.; Golu, I.; Taban, S.; Lazar, E.; Popescu, R.; Dobrescu, A.
- Abstract
Differentiating between benign and malignant tumors is essential for the management of thyroid nodules. This study aimed to appreciate the diagnostic value of a panel of molecular markers (CK19, HBME-1 and gal-3) in distinguishing various thyroid tumors. From a group of 123 clinically and paraclinically investigated thyroid lesions, we evaluated immunohistochemically (IHC) the tissue expression of the 3 markers in 41 cases, including: 2 cases of papillary hyperplasia (PH), 12 follicular adenomas (FA), 18 papillary carcinomas (PC), 5 follicular carcinomas (FC) and 4 tumors of uncertain malignant potential (TUMP). Deparaffined tissue sections were stained IHC (LSAB technique, visualization with DAB) using anti-CK19 (clone RCK 108), anti-mesothelial cell (HBME-1) and anti Gal-3 (clone 9C4) monoclonal antibodies; the data obtained was analyzed statistically using Fisher's test. CK19 was expressed in 99.44% of PC, in 60% of FC and 2 TUMP, being focal and weak in 41.6% of FA and occasionally in hyperplastic nodules (more evident in areas with degenerative changes). HBME-1 was expressed in PC (83%) and lymph node metastasis of PC, being absent in most of the other benign lesions. The panel made up of the three markers (CK19, HBME-1 and Gal-3) can be used as a supplement to morphologic criteria and it helps optimizing the management of patients with thyroid nodules. HBME-1 expressed predominantly in malignant lesions can be considered the most sensitive and specific marker in PC. Gal-3 was positive in 94% of PC; weak focal Gal-3 expression in the tissue surrounding the tumor and in benign lesions threw posed difficulties in immunoreaction interpretation.
- Subjects
IMMUNOHISTOCHEMISTRY; BIOMARKERS; THYROID gland tumors; PAPILLARY carcinoma; SQUAMOUS cell carcinoma; HYPERPLASIA; GENE expression; GENETICS
- Publication
Annals of the Romanian Society for Cell Biology, 2012, Vol 17, Issue 2, p43
- ISSN
2067-3019
- Publication type
Article