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- Title
表达PRRSV NADC30-like毒株GP5-M的重组伪狂犬病病毒的构建及其生物学特性探究.
- Authors
孙珊珊; 其美拉姆; 李强; 曾南方; 郑诚; 张白玉; 颜其贵
- Abstract
To obtain a recombinant pseudorabies virus PRV-GP5-M strain that can fuse and express the GP5-M protein of porcine reproductive and respiratory syndrome virus (PRRSV), using the porcine pseudorabies virus (PRV) variant TK gene deletion strain PRV FJ01/TK-as the viral vector and the gI/gE gene as the insertion target, the gI/gE gene was knocked out using homologous recombination technology and CRISPR/Cas9 technology, and a CMV-GP5-M expression box was inserted at the gI/gE position. After plaque purification, a recombinant virus PRV-GP5-M that could correctly express the GP5-M protein was successfully constructed. Furthermore, the biological characteristics of the strain, including stability, growth kinetics, culture characteristics and safety were explored. The results showed that the recombinant strain had good genetic stability, safety, and was easy to proliferate and cultivate. This study result provided new clues for targeting PRRSV GP5 and M protein to generate new PRRSV vaccines, and provided important references for vaccines against the prevalent PRRSV NADC30-like and PRV variant strains in recent years.
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 11, p2555
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.20221629