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- Title
患白内障病黑斑蛙米尔伊丽莎白菌的分离鉴定与 PVCase 基因克隆.
- Authors
龚保荣; 吴红军; 李本镇; 徐大洋; 邹文腾; 曲君艺; 鲍传和; 朱若林
- Abstract
In order to provide treatment protocols for the cataract disease of black-spotted pond frog (Pelophylax nigromaculatus) in Shucheng area, Anhui Province, four dominant bacterial strains were isolated from the liver, eyes, spine and brain, respectively. The 16S rRNA gene and gyrB gene sequences of the isolated strains were analyzed, and the results showed that the four strains were Elizabethkingia miricola, which was confirmed by physiological and biochemical identification. N-glycosidase (PNGase) genes of the isolated strain was cloned and analysed, and the results showed it contained two types, PNGase F and PNGase F-Ⅱ. The phylogenetic analysis of PNGase F and PNGase F-Ⅱ both showed that the isolated strain was clustered with Elizabethkingia miricola in Elizabethkingia genus, which indicated that they can be used as reference genes for the classification of Elizabetha genus. Antimicrobial susceptibility test showed that the four isolates were only sensitive to florfenicol in amidols, intermediary to neosporin in coumarin, erythromycin in macrolide and rifampin in rifamycin, and resistant to other 14 antibiotics. The experiment results will provide a reference for the prevention and treatment of the bacterial diseases of black-spotted pond frog.
- Subjects
ANHUI Sheng (China); MICROBIAL sensitivity tests; CATARACT; BACTERIAL diseases; RIFAMYCINS; MEDICAL protocols; PEPTIDE antibiotics
- Publication
Acta Agriculturae Zhejiangensis, 2023, Vol 35, Issue 6, p1297
- ISSN
1004-1524
- Publication type
Article
- DOI
10.3969/j.issn.1004-1524.2023.06.07