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- Title
T,B淋巴细胞自噬在自身免疫性肝炎患者外周血中的 表达及临床意义.
- Authors
伍慧丽; 时红波; 刘燕敏; 严 艳; 王玲玲; 丁 美; 马正来; 刘 晖; 段钟平
- Abstract
Objective To investigate the expression of autophagy marker in peripheral blood T and B lymphocytes of patients with autoimmune hepatitis(AIH) and its clinical significance. Methods Peripheral blood samples were collected from 62 AIH patients who were treated in Beijing YouAn Hospital affiliated to Capital Medical University from October 2019 to October 2020 who were treated in Beijing YouAn Hospital affiliated to Capital Medical University from October 2019 to October 2020 and 8 healthy controls to detect autophagy of T and B lymphocyte subsets, and then subgroup analyses were performed based on treatment, diagnostic type, and presence or absence of liver cirrhosis and liver failure. The t-test was used for comparison of normally distributed continuous data between two groups; the Kruskal-Wallis H test was used for comparison of non-normally distributed continuous data between multiple groups, and the Mann-Whitney U test was used for comparison between two groups; the chi-square test or the Fisher’s exact test was used for comparison of categorical data between two groups. Results Compared with the healthy control group, the AIH group had a significantly higher mean fluorescence intensity(MFI) of the autophagy marker LC3 B in CD4+ T, CD8+ T, CD19+ B, and CD4+ CD25+ T lymphocytes(all P<0.05), especially in CD19+ B lymphocytes. The non-treatment group and the partial remission group had a significantly higher MFI of autophagy marker in CD19+ B lymphocytes than the complete remission group(P=0.037 and 0.040); the idiopathic AIH(I-AIH) group and the drug-induced AIH(DI-AIH) group had a significantly higher MFI than the primary biliary cholangitis(PBC)-AIH overlap syndrome group(P=0.037 and 0.031); the non-cirrhosis group and the decompensated cirrhosis group had a significantly higher MFI than the compensated cirrhosis group(P=0.009 and 0.003); the liver failure group had a significantly higher MFI than the non-liver failure group(P=0.042). The PBC-AIH group had a significantly higher MFI of autophagy marker in CD4+ CD25+ T lymphocytes than the I-AIH group and the DI-AIH group(P=0.042 and 0.044), the compensated cirrhosis group had a significantly lower MFI than the non-cirrhosis group(P=0.037), and the non-liver failure group had a significantly higher MFI than the liver failure group(P=0.043). Conclusion AIH patients have a significant increase in the expression of autophagy marker in peripheral blood T and B lymphocyte subsets compared with healthy individuals, and the level of autophagy is associated with treatment, diagnostic type, and disease severity.
- Publication
Journal of Clinical Hepatology / Linchuang Gandanbing Zazhi, 2022, Vol 38, Issue 11, p2483
- ISSN
1001-5256
- Publication type
Article
- DOI
10.3969/j.issn.1001-5256.2022.11.009