We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
CRISPR Technology Reveals RAD(51)-ical Mechanisms of Repair in Roundworms: An Educational Primer for Use with ?Promotion of Homologous Recombination by SWS-1 in Complex with RAD-51 Paralogs in Caenorhabditis elegans?
- Authors
Turcotte, Carolyn A.; Andrews, Nicolas P.; Sloat, Solomon A.; Checchi, Paula M.
- Abstract
The mechanisms cells use to maintain genetic fidelity via DNA repair and the accuracy of these processes have garnered interest from scientists engaged in basic research to clinicians seeking improved treatment for cancer patients. Despite the continued advances, many details of DNA repair are still incompletely understood. In addition, the inherent complexity of DNA repair processes, even at the most fundamental level, makes it a challenging topic. This primer is meant to assist both educators and students in using a recent paper, ?Promotion of homologous recombination by SWS-1 in complex with RAD-51 paralogs in Caenorhabditis elegans,? to understand mechanisms of DNA repair. The goals of this primer are to highlight and clarify several key techniques utilized, with special emphasis on the clustered, regularly interspaced, short palindromic repeats technique and the ways in which it has revolutionized genetics research, as well as to provide questions for deeper in-class discussion.
- Subjects
CRISPRS; DNA repair; RAD51 recombinase; CAENORHABDITIS elegans; MEIOSIS
- Publication
Genetics, 2016, Vol 204, Issue 3, p883
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.116.195479