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- Title
DREF is required for EGFR signalling duringDrosophilawing vein development.
- Authors
Yoshida, Hideki; Kwon, Eunjeong; Hirose, Fumiko; Otsuki, Kyoko; Yamada, Mikihiro; Yamaguchi, Masamitsu
- Abstract
The DNA replication-related element binding factor (DREF) has been suggested as being involved in regulation of DNA replication- and proliferation-related genes inDrosophila. Recently, by searching theDrosophilagenome database, we also found DRE-like sequences in the 5′-flanking regions of many genes with other functions. In addition, immunostaining of polytene chromosomes with an anti-DREF monoclonal antibody revealed that DREF can bind to a hundred regions of polytene chromosomes, suggesting regulation of multiple genes and multiple rolesin vivo. When we over-expressed DREF protein or inverted repeat RNA of theDREFgene in wing imaginal discs using the GAL4-UAS targeted expression system inDrosophila, the results were veins of increased width and a loss of veins, respectively. With DREF over-expression, Rolled, aDrosophilaMAPK homologue, was ectopically activated. Furthermore, half reduction of theD-rafgene dose suppressed this DREF-induced vein of increased width phenotype. In addition, when DREF transcripts were reduced by introducing double-stranded RNA of theDREFgene into S2 cells, theD-rafgene promoter activity was diminished to 4%. These data indicate that DREF is involved in regulation of vein formation through the activation of EGFR signalling in theDrosophilawing imaginal discs.
- Subjects
BLOOD vessels; DNA replication; CELL nuclei; MOLECULAR cloning; MONOCLONAL antibodies; INTERFERON inducers
- Publication
Genes to Cells, 2004, Vol 9, Issue 10, p935
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1111/j.1365-2443.2004.00775.x