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- Title
Autoregulation of ZEB 2 expression for zearalenone production in F usarium graminearum.
- Authors
Park, Ae Ran; Son, Hokyoung; Min, Kyunghun; Park, Jinseo; Goo, Jae Hwan; Rhee, Sangkee; Chae, Suhn‐Kee; Lee, Yin‐Won
- Abstract
Several F usarium species produce the polyketide mycotoxin zearalenone ( ZEA), a causative agent of hyperestrogenic syndrome in animals that is often found in F . graminearum-infected cereals in temperate regions. The ZEA biosynthetic cluster genes PKS 4, PKS 13, ZEB 1 and ZEB 2 encode a reducing polyketide synthase, a non-reducing polyketide synthase, an isoamyl alcohol oxidase and a transcription factor respectively. In this study, the production of two isoforms ( ZEB2 L and ZEB2 S) from the ZEB 2 gene in F . graminearum via an alternative promoter was characterized. ZEB2 L contains a basic leucine zipper (b ZIP) DNA-binding domain at the N-terminus, whereas ZEB2 S is an N-terminally truncated form of ZEB2 L that lacks the b ZIP domain. Interestingly, ZEA triggers the induction of both ZEB 2 L and ZEB 2 S transcription. ZEB2 L and ZEB2 S interact with each other to form a heterodimer that regulates ZEA production by reducing the binding affinity of ZEB2L for the ZEB2 L gene promoter. Our study provides insight into the autoregulation of ZEB 2 expression by alternative promoter usage and a feedback loop during ZEA production; this regulatory mechanism is similar to that observed in higher eukaryotes.
- Subjects
ZEARALENONE; FUSARIUM; FUNGAL gene expression; ANIMAL species; POLYKETIDE synthases
- Publication
Molecular Microbiology, 2015, Vol 97, Issue 5, p942
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.13078