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- Title
A Pilot Study for the Mutation Assay Using a High-throughput DNA Sequencer.
- Authors
Tomonari Matsuda; Makiko Takamune; Yoko Matsuda; Masami Yamada
- Abstract
We present here a mutation assay with little bias which incorporates high-throughput DNA sequencing technology. Our strategy is simple: 1) expose cells to a test compound, 2) isolate colonies, and 3) carry out whole-genome sequencing of the clones. In this pilot study, we used Salmonella typhimurium TA100 as a tester strain and successfully detected mutations induced by the mutagen 2- (2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2). We believe that this new mutation assay will be a very useful tool in hazard assessment of chemicals.
- Subjects
GENETIC mutation; NUCLEOTIDE sequence; COLONIES (Biology); SALMONELLA typhimurium; ACRYLAMIDE; CLONING
- Publication
Genes & Environment, 2013, Vol 35, Issue 2, p53
- ISSN
1880-7046
- Publication type
Article
- DOI
10.3123/jemsge.35.53