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- Title
Microbial communities associated with the co-metabolism of free cyanide and thiocyanate under alkaline conditions.
- Authors
Mekuto, Lukhanyo; Ntwampe, Seteno Karabo Obed; Mudumbi, John Baptist N.
- Abstract
This study focused on the identification of free cyanide (CDO) and thiocyanate (TDO) degrading microbial communities using a culture-dependent and independent approach. Culturable microbial species were isolated from the CDOs (<italic>n</italic> = 13) and TDOs (<italic>n</italic> = 18). The CDOs were largely dominated by <italic>Bacillus</italic> sp. while the TDOs were dominated by <italic>Bacillus</italic> sp., <italic>Klebsiella oxytoca</italic>, <italic>Providencia</italic> sp. and <italic>Pseudomonas</italic> sp. However, 16S rRNA amplicon gene-sequencing revealed the complexity and diversity of the microbial communities in contrast to the organisms that were detected using culture-dependent technique. Overall, the organisms were mainly dominated by <italic>Myroides odoratimimus</italic> and <italic>Proteus</italic> sp. at 37.82 and 30.5% for CDOs, and 35.26 and 17.58% for TDOs, respectively. The co-culturing of the CDOs and TDOs resulted in biochemical changes of key metabolic enzymes, and this resulted in the complete degradation of CN− and SCN− simultaneously; a phenomenon which has not been witnessed, especially under alkaline conditions. Current ongoing studies are focused on the application of these organisms for the biodegradation of CN− and SCN− in a continuous system, under changing operational parameters, to assess their effectiveness in the biodegradation of CN− and SCN−.
- Subjects
MICROBIAL communities; CYANIDES; THIOCYANATES; KLEBSIELLA oxytoca; BIODEGRADATION
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1124-3